{"id":46,"date":"2025-03-14T19:22:37","date_gmt":"2025-03-14T11:22:37","guid":{"rendered":"https:\/\/zhanglab.top\/?page_id=46"},"modified":"2026-10-06T09:40:33","modified_gmt":"2026-10-06T01:40:33","slug":"publication","status":"publish","type":"page","link":"https:\/\/zhanglab.top\/en\/publication\/","title":{"rendered":"Representative Publications"},"content":{"rendered":"<ul class=\"wp-block-list\">\n<li>Reprogramming orthogonal regulatory and metabolic system for scalable production of secondary metabolites. <br><strong><em>Nature Chemical Engineering<\/em><\/strong>. 2026-09. (Accept)<\/li>\n\n\n\n<li>Scalable secondary metabolite production in <em>Streptomyces<\/em> using a plug-and-play system. <br><strong><em>Nature Biotechnology<\/em><\/strong>. 2025-08. DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41587-025-02762-1\">10.1038\/s41587-025-02762-1<\/a>.<\/li>\n\n\n\n<li>Orchestration of secondary metabolite production in <em>Streptomyces species<\/em>.<br><strong><em>Nature Biotechnology<\/em><\/strong>. 2025-09. DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41587-025-02762-1\">10.1038\/s41587-025-02762-1<\/a>.<\/li>\n\n\n\n<li>High-yield Porphyrin Production through Metabolic Engineering and Biocatalysis. <br><strong><em>Nature Biotechnology<\/em><\/strong>. 2024-06. DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41587-024-02267-3\">10.1038\/s41587-024-02267-3<\/a>.<\/li>\n\n\n\n<li>Harnessing the Intracellular Triacylglycerols for Titer Improvement of Polyketides in Streptomyces. <br><strong><em>Nature Biotechnology<\/em><\/strong>. 2019-12. DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41587-019-0335-4\">10.1038\/s41587-019-0335-4<\/a>.<\/li>\n\n\n\n<li>Profile and Relaxation of Sequence-Specificity of DNA Sulfur Binding Domains Facilitate New Nucleic Acid Detection Platform. <br><em><strong>Science Bulletin<\/strong><\/em>. 2023-08. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.scib.2023.07.012\">10.1016\/j.scib.2023.07.012<\/a>.<\/li>\n\n\n\n<li>Berberine Reverses Multidrug Resistance in Candida Albicans by Hijacking the Drug Efflux Pump Mdr1p. <br><strong><em>Science Bulletin<\/em><\/strong>. 2021-09. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.scib.2020.12.035\">10.1016\/j.scib.2020.12.035<\/a>.<\/li>\n\n\n\n<li>A Versatile Biosensing Platform Coupling CRISPR\u2013Cas12a and Aptamers for Detection of Diverse Analytes. <br><strong><em>Science Bulletin<\/em><\/strong>. 2021-01. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.scib.2020.09.004\">10.1016\/j.scib.2020.09.004<\/a>.<\/li>\n\n\n\n<li>Reprogramming ThDP Enzymes for\u00a0<em>Z<\/em>-Alkenes: Overriding Thermodynamic Preference via Noncovalent Controls. <br><strong><em>Journal of the American Chemical Society<\/em><\/strong>. 2026-06. DOI: <a href=\"https:\/\/doi.org\/10.1021\/jacs.6c06951\">10.1021\/jacs.6c06951<\/a>.<\/li>\n\n\n\n<li>Enzyme-Catalyzed Intramolecular C\u2013C Coupling Transformation of Nitriles.<br><strong><em>Journal of the American Chemical Society<\/em><\/strong>. 2026-02. DOI: <a href=\"https:\/\/doi.org\/10.1021\/jacs.6c02210\">10.1021\/jacs.6c02210<\/a>.<\/li>\n\n\n\n<li>Taming Highly Enolizable Aldehydes via Enzyme Catalysis for Enantiocomplementary Construction of \u03b2-Hydroxyphosphonates. <br><strong><em>Journal of the American Chemical Society<\/em><\/strong>. 2025-01. DOI: <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/jacs.4c11957\">10.1021\/jacs.4c11957<\/a>.<\/li>\n\n\n\n<li>Structural and mechanistic insights into azetidine-associated\u00a0<em>\u03b1<\/em>KG-NHFe enzyme OkaE with multifunctional catalysis<br><strong><em>Nature Communications<\/em><\/strong>. 2026-02. DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41467-026-69519-5\">10.1038\/s41467-026-69519-5<\/a>.<\/li>\n\n\n\n<li>A Thermostable Type IB CRISPR-Cas System for Orthogonal and Multiplexed Genetic Engineering. <br><em><strong>Nature Communications<\/strong><\/em>. 2023-10. DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41467-023-41973-5\">10.1038\/s41467-023-41973-5<\/a>.<\/li>\n\n\n\n<li>A CRISPR-Cas12a-Derived Biosensing Platform for the Highly Sensitive Detection of Diverse Small Molecules. <br><strong><em>Nature Communications<\/em><\/strong>. 2019-08. DOI: <a href=\"https:\/\/doi.org\/10.1038\/s41467-019-11648-1\">10.1038\/s41467-019-11648-1<\/a>.<\/li>\n\n\n\n<li>Model-Guided Systematic Metabolic Engineering for Enhanced Spinosad Biosynthesis in\u00a0<em>Saccharopolyspora spinosa<\/em>\u00a0NHF132. <br><strong><em>Advanced Science<\/em><\/strong>. 2025-09.\u00a0DOI: <a href=\"https:\/\/doi.org\/10.1002\/advs.202511411\">10.1002\/advs.202511411<\/a>.<\/li>\n\n\n\n<li>Harnessing a Previously Unidentified Capability of Bacterial Allosteric Transcription Factors for Sensing Diverse Small Molecules in Vitro. <br><strong><em>Science Advances<\/em><\/strong>. 2018-11. DOI: <a href=\"https:\/\/doi.org\/10.1126\/sciadv.aau4602\">10.1126\/sciadv.aau4602<\/a>.<\/li>\n\n\n\n<li>Function Switch of a Fungal Sesterterpene Synthase through Molecular Dynamics Simulation Assisted Alteration of an Aromatic Residue Cluster in the Active Pocket of PfNS. <br><strong><em>Angewandte Chemie International Edition<\/em><\/strong>. 2024-09. DOI: <a href=\"https:\/\/doi.org\/10.1002\/anie.202406246\">10.1002\/anie.202406246<\/a>.<\/li>\n\n\n\n<li>Development of an Escherichia coli-based diterpenoid bioproduction foundry. <br><strong><em>Chemical Engineering Journal<\/em><\/strong>. 2026-11. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.cej.2026.180407\">10.1016\/j.cej.2026.180407<\/a>.<\/li>\n\n\n\n<li>Expanding horizons of CRISPR applications beyond genome editing. <br><strong><em>Trends in Genetics<\/em><\/strong>. 2025-07. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.tig.2025.06.003\">10.1016\/j.tig.2025.06.003<\/a>.<\/li>\n\n\n\n<li>Systematic Development of a Highly Efficient Cell Factory for 5-Aminolevulinic Acid Production. <br><strong><em>Trends in Biotechnology<\/em><\/strong>. 2024-11. DOI: <a href=\"http:\/\/10.1016\/j.tibtech.2024.06.004\">10.1016\/j.tibtech.2024.06.004<\/a>.<\/li>\n\n\n\n<li>Unleashing the Potential: Type I CRISPR-Cas Systems in Actinomycetes for Genome Editing. <br><strong><em>Natural Product Reports<\/em><\/strong>. 2024-06. DOI: <a href=\"https:\/\/doi.org\/10.1039\/D4NP00010B\">10.1039\/D4NP00010B<\/a>.<\/li>\n\n\n\n<li>Anti-Mycobacterial Natural Products and Mechanisms of Action. <br><strong><em>Natural Product Reports<\/em><\/strong>. 2021-07. DOI: <a href=\"https:\/\/doi.org\/10.1039\/D1NP00011J\">10.1039\/D1NP00011J<\/a>.<\/li>\n\n\n\n<li>ETfinder: harnessing conserved C-terminal tails of single-stranded DNA-binding proteins for mining and engineering RecET systems in non-model microbial chassis. <br><strong><em>Nucleic Acids Research<\/em><\/strong>. 2026-08. DOI: <a href=\"https:\/\/doi.org\/10.1093\/nar\/gkag818\" data-type=\"link\" data-id=\"https:\/\/doi.org\/10.1093\/nar\/gkag818\">10.1093\/nar\/gkag818<\/a>.<\/li>\n\n\n\n<li>Activating Cryptic Biosynthetic Gene Cluster through a CRISPR\u2013Cas12a-Mediated Direct Cloning Approach. <br><strong><em>Nucleic Acids Research<\/em><\/strong>. 2022-03. DOI: <a href=\"https:\/\/doi.org\/10.1093\/nar\/gkac181\">10.1093\/nar\/gkac181<\/a>.<\/li>\n\n\n\n<li>Reporter systems in actinomycetes: Versatile tools for natural product discovery and production. <br><strong><em>Biotechnology Advances<\/em><\/strong>. 2026-05. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.biotechadv.2026.108831\">10.1016\/j.biotechadv.2026.108831<\/a>.<\/li>\n\n\n\n<li>Engineering the D\u2011Helix in Fungal Sesterterpene Synthase ZbSS Unlocks a Skeletal Rearrangement Cyclization Route toward Sesterterpene Diversification. <br><strong><em>ACS Catalysis<\/em><\/strong>. 2026-02. DOI: <a href=\"https:\/\/doi.org\/10.1021\/acscatal.5c07482\" target=\"_blank\" rel=\"noopener\">10.1021\/acscatal.5c07482<\/a>.<\/li>\n\n\n\n<li>G2 Helix as the Plastic Region Modulating Diastereoselectivity of Cyclization in Fungal Bifunctional Sesterterpene Synthases. <br><strong><em>ACS Catalysis<\/em><\/strong>. 2025-07. DOI: <a href=\"https:\/\/doi.org\/10.1021\/acscatal.5c03947\">10.1021\/acscatal.5c03947<\/a>.<\/li>\n\n\n\n<li>Access to Axially Chiral Biaryl Benzylamines via Ancestral Enzyme-Enabled Reductive Amination Desymmetrization. <br><strong><em>ACS Catalysis<\/em><\/strong>. 2025-01. DOI: <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acscatal.4c06881\">10.1021\/acscatal.4c06881<\/a>.<\/li>\n\n\n\n<li>Biochemical and Structural Characterization of OvoA<sub>Th2<\/sub>: A Mononuclear Nonheme Iron Enzyme from Hydrogenimonas thermophila for Ovothiol Biosynthesis. <br><strong><em>ACS Catalysis<\/em><\/strong>. 2023-11. DOI: <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acscatal.3c04026\">10.1021\/acscatal.3c04026<\/a>.<\/li>\n\n\n\n<li>Hydrogenase as the Basis for Green Hydrogen Production and Utilization. <br><strong><em>Journal of Energy Chemistry<\/em><\/strong>. 2023-10. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.jechem.2023.06.018\">10.1016\/j.jechem.2023.06.018<\/a>.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">&#8230;<\/p>","protected":false},"excerpt":{"rendered":"<p>&#8230;<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-46","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/pages\/46","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/comments?post=46"}],"version-history":[{"count":33,"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/pages\/46\/revisions"}],"predecessor-version":[{"id":259,"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/pages\/46\/revisions\/259"}],"wp:attachment":[{"href":"https:\/\/zhanglab.top\/en\/wp-json\/wp\/v2\/media?parent=46"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}